|Immunohistochemistry (Frozen) (IHC (F))||1:40-1:80|
|Immunohistochemistry (Paraffin) (IHC (P))||1:40-1:80|
|Immunoprecipitation (IP)||10 µl/mg lysate|
|Western Blot (WB)||1:50-1:100|
|Immunohistochemistry (IHC)||See 22 publications below|
|Western Blot (WB)||See 2 publications below|
|Gel Shift (GS)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 6 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rabbit , Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant estrogen receptor protein (alpha form)|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-27107 detects estrogen receptor alpha from human and rat samples.
MA1-27107 has successfully been used in IHC, IP, and Western blot procedures. Paraffin sections require an antigen retrieval pretreatment with sodium citrate buffer (pH 6.0) prior to staining. Use Samboni and quote;s fixative for optimal staining results. Positive control of Breast carcinoma suggested.
The MA1-27107 immunogen is recombinant human estrogen receptor alpha.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; ER Alpha; ER-alpha; ESR1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: ER; ER-alpha; Era; ESR; ESR1; ESRA; Estr; ESTRR; NR3A1; RNESTROR
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