|Immunofluorescence (IF)||1:100 -1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500 - 1:2000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rabbit|
|Published species reactivity||Rabbit|
|Host / Isotype||Mouse|
|Immunogen||Mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) and E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-310 detects estrogen receptor alpha in human and rabbit samples.
MA3-310 has been successfully used in immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects a ~68 kDa protein representing estrogen receptor alpha in MCF-7 cells, human breast tumors, and rabbit vaginal tissue. In immunohistochemistry this antibody recognizes the estrogen receptor alpha in MCF-7 cells, human breast tissue, and human smooth muscle cells.
The MA3-310 immunogen is a mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) and E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.
The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; ER Alpha; ER-alpha; ESR1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha-like; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
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