|Immunohistochemistry (Frozen) (IHC (F))||1-5 µg/ml|
|Western Blot (WB)||1 µg/ml|
|Immunohistochemistry (IHC)||See 5 publications below|
|Western Blot (WB)||See 7 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rat Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining.
The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. # PEP-036) is available for use in neutralization and control experiments.
Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.
The Estrogen Receptor alpha (ER alpha) protein is approximately 66kD, though multiple isoforms ranging from 36-66kD have been described. The ER alpha gene (ESR1) consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. Each domain is functionally distinct, required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; ER Alpha; ER-alpha; ESR1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: ER; ER-alpha; Era; ESR; ESR1; ESRA; Estr; ESTRR; NR3A1; RNESTROR
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