|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/5-1/50|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Tested Species reactivity||Camelid, Human, Non-human primate, Sheep, Pig, Reptile, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide derived from the C terminus of the human estrogen receptor beta 1 isoform, CEDSKSKEGSQNPQSQ.|
|Storage buffer||tissue culture supernatant|
|Contains||0.1% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
In immunohistochemical studies, this antibody has shown positive nuclear staining in cells within the ovary, uterus, oviduct, breast, testis, prostate, bladder and male reproductive duct. There are also reports of cell staining in the adenohypophysis, demonstrating cytoplasmic expression.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) or 0.05M glycine/EDTA (pH 8) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is ovary.
The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; PMPMEase; steroid receptor
Gene Aliases: ER-BETA; ERB; Erb2; ERBETA; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
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