|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(467) C S T E D S K N K E G S Q N L Q S Q(485) of mouse ER beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-312 detects estrogen receptor (ER) beta from rat, human, and mouse samples.
PA1-312 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat cerebellum with PA1-312 results in staining of cells in the paraventricular nucleus and retrochiasmatic nucleus.
The PA1-312 immunogen is a synthetic peptide corresponding to residues C(467) C S T E D S K N K E G S Q N L Q S Q(485) of mouse ER beta.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER Beta; ER-beta; ESR-Beta; ESR2; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta
Gene Aliases: ER-BETA; ER[b]; Erb; Erb2; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.