|Immunohistochemistry (Frozen) (IHC (F))||2-5 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2-5 µg/mL|
|Western Blot (WB)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Gel Shift (GS)||See 1 publications below|
|Western Blot (WB)||See 7 publications below|
|Immunohistochemistry (IHC)||See 11 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rat , Pig , Non-human primate , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-313 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat prostate with PA1-313 results in nuclear staining, whereas nuclear staining may not be observed in human tissue.
The PA1-313 immunogen is a synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta. PA1-313 immunizing peptide (Cat. # PEP-029) is available for use in neutralization and control experiments.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
The Estrogen Receptor beta (ER beta) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. The size and structure of ER beta is very similar to ER alpha, with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as the transactivation domain has diverged significantly. Similar in function to ER alpha, ER beta binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER Beta; ER-beta; ESR-Beta; ESR2; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2
Gene Aliases: ER-BETA; Erb; Erb2; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
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