|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1-3 µg/mL|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the residues M(1) S S Q V V G I E P L Y I K A E(16) of human ERR1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-314 detects human ERR1 from transfected cells and shows no cross-reactivity to ERR3.
PA1-314 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~52 kDa protein representing ERR1 from COS-7 transfected cells. An unidentified band is seen at ~37 kDa.
The PA1-314 immunogen is a synthetic peptide corresponding to the residues M(1) S S Q V V G I E P L Y I K A E(16) of human ERR1. This sequence is completely conserved between canine, human, mouse and rat. This immunizing peptide (PEP-231) can be used for neutralization and control experiments.
Estrogen-related receptor alpha 1 (ERR alpha, ERR1) is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members share an almost identical DNA-binding domain, which has 68% amino acid identity with that of estrogen receptor.
This protein binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-tnaaggtca-3' can bind to the medium-chain acyl coenzyme a dehydrogenase (mcad) response element nrre-1 and may act as an important regulator of mcad promoter. Studies have shown that ERR1 functions a metabolic regulator and is often expressed in tissues that preferentially metabolize fatty acids.
The corresponding gene for the ERR1 is NR3B1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ERR alpha; ERR-alpha; Estrogen receptor-like 1; estrogen-related nuclear receptor alpha; Estrogen-related receptor alpha; Estrogen-Related Receptor Alpha 1; estrogen-related receptor, alpha; Nuclear receptor subfamily 3 group B member 1; orphan nuclear receptor; Steroid hormone receptor ERR1
Gene Aliases: ERR1; ERRa; ERRalpha; Errra; ESRL1; ESRRA; NR3B1
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