|Immunohistochemistry (IHC)||2.5-5 µg/ml|
|Western Blot (WB)||1:10,000-1:20,000|
|Tested Species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human FANCD2 fusion protein (N-terminal fragment).|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
In Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form).
Additional bands may be seen at lower molecular weights.
In immunofluorescence, this has been tested in human MMC and IR-treated MEF cells.
Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANCD2, targeting nuclear foci containing the BRCA1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FA-D2; FA4; FACD; FAD; FAD2; FANCD; Fanconi anemia group D2 protein; Fanconi anemia group D2 protein homolog; Fanconi anemia, complementation group D2; Protein FACD2
Gene Aliases: 2410150O07Rik; AU015151; BB137857; FA-D2; FA4; FACD; FAD; FAD2; FANCD; FANCD2
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