|ELISA (ELISA)||0.5-1.0 ug/ml|
|Inhibition Assays (IA)||10-20 ug/ml|
|Neutralization (Neu)||10-20 ug/ml|
|Western Blot (WB)||0.1-0.2 ug/ml|
|Tested Species reactivity||Cat, Human, Mouse, Pig|
|Host / Isotype||Rat / IgG1|
|Immunogen||Purified, recombinant mouse Fas ligand (residues 132-279).|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-24729 detects Fas Ligand from mouse samples. MA1-24729 is expected to cross react with human (100% conserved), feline (89% conserved), and porcine (88% conserved) due to sequence homology.
MA1-24729 has been successfully used in neutralization, inhibition assays, and Western blot procedures. The Neutralization Dose50 (ND50) for Monoclonal Anti-Mouse Fas Ligand is approximately 10-20 ug/ml.
The MA1-24729 immunogen is purified, recombinant mouse Fas ligand (residues 132-279).
Fas ligand (FasL, CD 95L) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas (CD 95, Apo-1) is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family.
As a member of the TNF-cytokine family FasL induces apoptosis when interacting with its receptor Fas. FasL may exist as either membrane bound (45 kD) or soluble forms (26 kD).
The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of FasL to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins.
FasL is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. FasL expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney.
The Fas/FasL system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer. It is assumed that induction of apoptosis through FasL is predominantly involved in anti-viral immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APO1; apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; apoptosis antigen ligand 1; APTL; CD178; CD95 ligand; CD95-L; CD95L; Fas antigen ligand; Fas Ligand; Fas ligand (TNF superfamily, member 6); Fas-LG; FasL; generalized lymphoproliferative disease; mutant tumor necrosis factor family member 6; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand 1A; Tumor necrosis factor ligand superfamily member 6
Gene Aliases: ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; Fas-L; FASL; FASLG; gld; TNFSF6; TNLG1A
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.