|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from near N-terminus of human FAS ligand.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is testis, prostate or tonsil tissue.
FAS ligand is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where FAS ligand is expressed predominantly on activated T cells. FAS and FAS ligand are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APO1; apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; apoptosis antigen ligand 1; APTL; CD178; CD95 ligand; CD95-L; CD95L; Fas antigen ligand; Fas Ligand; Fas ligand (TNF superfamily, member 6); Fas-LG; FasL; mutant tumor necrosis factor family member 6; tumor necrosis factor ligand 1A; Tumor necrosis factor ligand superfamily member 6
Gene Aliases: ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; FASLG; TNFSF6; TNLG1A
UniProt ID: (Human) P48023
Entrez Gene ID: (Human) 356
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