|Flow Cytometry (Flow)||Assay-dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100-1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 39-56 of Human FGFR 4.|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-31991 detects FGFR4 from human samples.
PA1-31991 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot and ELISA applications. Detects a band of ~96 kDa. A suggested positive control for this product is THP-1 cell lysate.
The PA1-31991 immunogen is a synthetic peptide conjugated to KLH, corresponding to amino acids 39-56 of Human FGFR 4.
Store at 4°C for short term. For extended storage aliquot and store at -20°C or below. Avoid Freeze/Thaw Cycles.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD334; FGF Receptor 4; FGFR-4; Fibroblast Growth Factor Receptor 4; hydroxyaryl-protein kinase; JTK2; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase
Gene Aliases: CD334; FGFR4; JTK2; TKF
UniProt ID: (Human) P22455
Entrez Gene ID: (Human) 2264
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