|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:2500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:2500|
|Western Blot (WB)||1:1000-1:5000|
|Tested Species reactivity||Chemical, Tag|
|Host / Isotype||Goat / IgG|
|Immunogen||Fluorescein, conjugated to goat IgG.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-26804 detects fluorosceinated proteins and nucleic acids.
PA1-26804 has been successfully used in ELISA, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot procedures.
The PA1-26804 immunogen is Fluorescein, conjugated to goat IgG.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Fluorescein; Fluorescein isothiocyanate
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