|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:5000|
|Immunoprecipitation (IP)||10 ug/ml|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Bovine, Guinea pig, Hamster, Human, Mink, Mouse, Non-human primate, Rabbit|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified 9S rabbit uterine progesterone receptor.|
|Storage buffer||PBS, pH 7.2, with 50% glycerol|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This affinity purified antibody detects an ~59 kDa protein corresponding to the apparent molecular mass of FKBP59 on SDS-PAGE immunoblots in samples from human, monkey, bovine, mink, rabbit, hamster and guinea pig (weakly) origins. An additional, unidentified 50kDa band is observed in some cell lysates. This antibody has been shown to block the ability of FK506 to promote neurite outgrowth from SH-SY5Y cells in a concentration-dependent manner between 50 and 100nM. MA1-111 has also been successfully used in immunohistochemistry (paraffin) applications.
This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 51 kDa FK506-binding protein; 52 kDa FK506-binding protein; 52 kDa FKBP; 59 kDa FK506-binding protein 4; 59 kDa immunophilin; FK506 binding protein 4, 59kDa; FK506-binding protein 4; FK506-binding protein 4 (59kD); FKBP-4; FKBP51; FKBP52 protein; FKBP59; HBI; HSP binding immunophilin; HSP-binding immunophilin; Hsp56; hsp90 binding protein; Immunophilin FKBP52; p59; p59 protein; Peptidyl-prolyl cis-trans isomerase FKBP4; peptidylprolyl cis-trans isomerase; PPIase FKBP4; Rotamase; T-cell FK506-binding protein; T-cell FK506-binding protein, 59kD
Gene Aliases: 59kDa; AL022792; AW208983; BOS_6048; FKBP-4; FKBP-52; FKBP4; FKBP51; FKBP52; FKBP59; FKPB52; HBI; Hsp56; I79_001345; p52; P59; PPIase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.