|Western Blot (WB)||1:500 - 1:2000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide (DYKDDDDK) coupled to KLH.|
|Storage conditions||4° C|
similar epitope as the FLAG tag from Sigma
MA1-91878 detects Flag tag from various samples.
MA1-91878 has been successfully used in Western blot applications.
The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG Tag
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