|ChIP assay (ChIP)||1-3 µg x 10^6 cells|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.2 µg/ml|
|Western Blot (WB)||0.2 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Hamster, Human, Mouse|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide corresponding to residues M(1) M A S Y P E P E D T A G T(14) of mouse FoxL2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-802 detects FoxL2 protein in mouse and hamster samples.
PA1-802 has successfully been used in Western blot , immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~39 kDa protein representing FozL2 in CHO whole cell lysate.
The PA1-802 immunogen is a synthetic Peptide corresponding to residues M(1) M A S Y P E P E D T A G T(14) of mouse FoxL2. This sequence is completely conserved in mouse and rat and 92% conserved in human. This peptide (Cat. # PEP-238) is available for use in neutralization and control experiments.
FOXL2 is a winged helix/forkhead transcription factor that is highly conserved in human, goat, mouse, and certain aquatic species. Given its cross-species conservation, it appears to play a critical role evolutionarily, likely in ovarian differentiation in mammals and in ovarian somatic cell differentiation and in further follicle development and/or maintenance. Immunohistochemical data shows that FOXL2 is a nuclear protein specifically expressed in eyelids and in fetal and adult ovarian follicular cells. It does not undergo any major posttranslational modifications.
FOXL2 is also found to be associated with blepharo-phimosis/ptosis/epicanthus inverses syndrome (BPES). There are two forms of BPES. Type I BPES shows eyelid abnormal-ities that are associated with ovarian failure. In type II BPES, only eyelid defects are found. Studies have shown that FOXL2 is expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: forkhead box L2; Forkhead box protein L2; forkhead transcription factor FOXL2; P-Frk; Pituitary forkhead factor; putative transcription factor foxl2
Gene Aliases: AU045128; BPES; BPES1; FOXL2; PFRK; PINTO; POF3
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