|Flow Cytometry (Flow)||1:8000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:2000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide containing amino acids 43-100 of mouse FOXP3|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage conditions||-20°C or -80°C if preferred|
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: mouse CD4+CD25+ T cells.
FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.FOXP3 is stably and constitutively expressed at a high level in CD25+CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positibe T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; JM2; RP23-54C14.1; Scurfin; scurfy; sf
Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; scurfin; sf; XPID
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