|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Western Blot (WB)||1:1000-1:5000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431).|
|Purification||Antigen affinity chromatography|
|Storage buffer||sodium borate|
|Contains||0.05% sodium azide|
|Storage conditions||4° C, store in dark|
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AIID; DIETER; enteropathy; Forkhead box protein P3; FOXP3delta7; immune dysregulation; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; PIDX; polyendocrinopathy; Scurfin; scurfy; X-linked; XPID
Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; scurfin; sf; XPID
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.