|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: P(352) Q E E E K L S Q R N A N P G I K C(369)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-752 detects Fe65 from purified mouse samples. This antibody does not detect endogenous levels of Fe65.
PA1-752 has been successfully used in Western blot procedures. By Western blot, this antibody detects an 97 kDa protein representing Fe65 from immunopurified mouse brain lysate.
The PA1-752 immunizing peptide corresponds to amino acid residues 352-369 from mouse Fe65. This peptide (Cat. # PEP-200) is available for use in neutralization and control experiments.
The major transcript of the Fe65 gene is a neuron-specific mRNA that encodes a nuclear protein whose amino-terminal domain strongly activates the transcription of reporter genes. Fe65 has been implicated in a regulatory and cell signaling mechanism because it contains two different motifs involved in protein binding; a WW domain (a variant of Src homology 3 domains) and a phosphotyrosine interaction domain (PID). Interestingly, the PID domain binds to the same motif present in the conserved cytoplasmic domains of the beta PP and beta PP-like proteins.
RNA analyses revealed that Fe65 is predominantly expressed in the brain and in the regions most affected by Alzheimer and quote;s disease-associated neuropathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); Amyloid beta A4 precursor protein-binding family B member 1; APBB1; Protein Fe65; stat-like protein
Gene Aliases: APBB1; FE65; MGC:9072; RIR
Molecular Function: signaling molecule
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