|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Luciferase|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Luciferase protein from Photinus pyralis|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
MA1-90934 detects Luciferase from Bacteria, Invertebrates and Firefly samples.
MA1-90934 has been successfully used in Western blot applications.
The MA1-90934 immunogen is: Luciferase protein from Photinus pyralis
Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. It is known for its role as a reporter protein and can be used to quantify expression levels from promoters in tissue culture and in vivo. Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. Bioluminescence assay systems have become increasingly used in biology and medical research laboratories in addition to (or as alternatives to) fluorescence and chemiluminescence detection strategies. Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; Luciferin 4-monooxygenase; Photinus pyralis
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