|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Luciferase|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Luciferase protein isolated from Photinus pyralis.|
|Contains||0.08% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-12556 detects Luciferase in C. elegans and Drosophilia melanogaster tissue, human fibroblasts, mouse macrophages, kidney, liver and cortex tissues, as well as NIH-3T3, Jurkat and BHR21 cell lines with little to no background signal.
Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. It is known for its role as a reporter protein and can be used to quantify expression levels from promoters in tissue culture and in vivo. Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. Bioluminescence assay systems have become increasingly used in biology and medical research laboratories in addition to (or as alternatives to) fluorescence and chemiluminescence detection strategies. Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; Luciferin 4-monooxygenase; Photinus pyralis
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