|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500-1:1000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 11-25 of human Fis1.|
|Storage buffer||whole serum diluted in PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody is predicted to react with rat, chimp, and canine based on sequence homology.
Suggested positive control: antigen standard for FIS1 (transient overexpression lysate), 3T3 or Hela whole cell lysate.
Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CGI-135; FIS1 homolog; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11; TTC11
Gene Aliases: 2010003O14Rik; CGI-135; FIS1; TTC11
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