|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Western Blot (WB)||1:500-1:2000|
|Immunocytochemistry (ICC)||See 15 publications below|
|Blocking Assay (BLOCK)||See 3 publications below|
|Western Blot (WB)||See 7 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Mouse|
|Published species reactivity||Dog , Rat , Pig , Non-human primate , Hamster , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-062 detects furin convertase from canine and mouse cells as well as transfected human furin. This antibody does not detect endogenous furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells, or from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.
PA1-062 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects an ~100 kDa protein representing furin in BSC-40 cells transfected with the human furin gene. In addition, PA1-062 detects human furin transfected into 3T3 cells. Immunofluorescence staining of endogenous furin convertase in 3T3 cells with PA1-062 yields a pattern consistent with paranuclear staining.
The PA1-062 immunogen is a synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin. The PA1-062 immunizing peptide (Cat. # PEP-010) is available for use in neutralization and control experiments.
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 9130404I01Rik; dibasic processing enzyme; Dibasic-processing enzyme; FES upstream region; Fur; Furin; furin (paired basic amino acid cleaving enzyme); furin, membrane associated receptor protein; PACE; paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; Pcsk3; Prohormone convertase 3; proprotein convertase subtilisin/kexin type 3; SPC1; subtilisin pro-protein processing enzyme
Gene Aliases: 9130404I01Rik; FUR; FURIN; PACE; PCSK3; SPC1
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