|Western Blot (WB)||0.5 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH-conjugated, corresponding to a portion of amino acids 1-100 of human GaD67.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
The sequence used is 100% homologous with mouse and 94% homologous with rat GaD67.
Suggested positive control: antigen standard for GAD1 (transient overexpression lysate), SK-N-SH.
GAD67, a 67KDa protein, is a neuronal isoform of this enzyme predominantly expressed in human Brain. It consists of Pyridoxal 5 and quote;-phosphate binding site and conserved Cysteine residues which are vital for its activity and is inhibited due to phosphorylation. It has high binding affinity for the cofactor Pyridoxal-5 and quote;phosphate and its role has been implicated in normal Palate development. It is found to be associated with childhood onset schizophrenia and craniofacial malformations and has been identified as a major auto antigen and an auto-reactive T-cell target in IDDM. Deficiency of this enzyme leads to pyridoxine dependency along with epilepsy. It also might play a role in stiff main syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform; Glutamic acid decarboxylase 67
Gene Aliases: CPSQ1; GAD; GAD1; GAD67; SCP
UniProt ID: (Human) Q99259
Entrez Gene ID: (Human) 2571
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