|Immunofluorescence (IF)||1:50 - 1:100|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to amino acids C R(595) I T Q A Q E E L S P C L T P A A R(612) from human GADD34|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-139 detects an ~73 kDa protein in HeLa cells. PA1-139 shows specificity to GADD34 and can be blocked by incubating with an abundance of the immunizing peptide.
Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response gene 116; Myeloid differentiation primary response protein MyD116; Myeloid differentiation primary response protein MyD116 homolog; PPP1R15A; PR15A; Protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A; protein phosphatase 1, regulatory subunit 15A
Gene Aliases: 9630030H21; GADD34; Myd116; PPP1R15A
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