|Western Blot (WB)||1:1000-1:4000|
|Western Blot (WB)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||Non-human primate, Sheep, Pig, Rat, Zebrafish, Avian, Bovine, Hamster, Human, Marsupial, Mouse|
|Published species reactivity||Bovine Human|
|Host / Isotype||Mouse / IgM, kappa|
|Immunogen||Extensively purified pig GAPDH.|
|Contains||10mM sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody is likely to react with most mammals.
Suggested positive control: antigen standard for GAPDH (transient overexpression lysate).
GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; bb02e05; epididymis secretory sperm binding protein Li 162eP; G3PD; G3PDH; GAPD; GAPDH; glyceraldehyde phosphate dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
Gene Aliases: BARS-38; BOS_5957; cb609; CDABP0047; G3PD; G3PDH; GAPD; GAPDH; HEL-S-162eP; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:ft80f05
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