|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1:1000 - 1:10,000|
|Western Blot (WB)||See 109 publications below|
|Miscellaneous PubMed (MISC)||See 7 publications below|
|Tested Species reactivity||Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast|
|Published species reactivity||Yeast , Rabbit , Rat , Pig , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15738 detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.
MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS and ELISA.
MA5-15738 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; GAPDH; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; GAPDH; HEL-S-162eP; KNC-NDS6; OK/SW-cl.12
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