|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000 - 1:3000|
|Immunocytochemistry (ICC)||See 5 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Western Blot (WB)||See 12 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15256 recognizes native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP.
MA5-15256 has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications.
MA5-15256 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: eGFP; GFP; GFP2; Green Fluoresecent Protein; Turbo GFP
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