|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human GNB-2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is placenta tissue.
Guanine nucleotide binding proteins (G proteins) are membrane associated, heterotrimeric proteins composed of three subunits: alpha, beta and gamma. G proteins and their receptors (GPCRs) form one of the most prevalent signalling systems in mammalian cells, regulating systems as diverse as sensory perception, cell growth and hormonal regulation.
The G protein beta subunit assumes a barrel shaped beta propeller structure containing WD40 repeats preceded by an N terminal alpha helix. The beta subunit forms a stable dimer with the gamma subunit. The alpha subunit only contacts the beta subunit in the dimer, lying on the opposite face from the gamma subunit. RGS proteins that contain GGL (G protein gamma like) domains can interact with beta subunits to form novel dimers that prevent gamma subunit binding, and may prevent heterotrimer formation by inhibiting alpha subunit binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta-2 subunit; G protein; G protein subunit beta-2; G protein, beta-2 subunit; guanine nucleotide binding protein (G protein), beta polypeptide 2; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 2; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2; guanine nucleotide-binding protein GI/GS/GT beta subunit 2; guanine nucleotide-binding protein GI/GS/GT subunit beta-2; signal-transducing guanine nucleotide-binding regulatory protein beta subu; signal-transducing guanine nucleotide-binding regulatory protein beta subunit; Transducin beta chain 2
Gene Aliases: GNB2
UniProt ID: (Human) P62879
Entrez Gene ID: (Human) 2783
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