|Immunohistochemistry (Paraffin) (IHC (P))||1:3000|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated Cys-RKSFRSGSLRSLSNINSEML|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
IHC (P) analysis shows positive staining of GPR18 in human enteric ganglion tissue.
WB analysis shows GPR18 in glycoprotein-enriched fractions from GPR18 overexpressing 293 cells. A band of similar MW was also detected in GPR3 overexpressing 293 cells, suggesting some cross-reactivity with GPR3 or enhanced detection of basal GPR18 in the enriched fractions.
While GPR18 has a theoretical MW of ~ 38kD, the observed band in a WB runs at ~70kD due to enrichment of the glycosylated receptor.
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. GPR18 is an orphan G protein-coupled receptor found at high levels in the testis, small intestines and cells associated with the immune system including lymphocytes, thymus and spleen. GPR18 has been found to be a receptor for endogenous lipid neurotransmitters, several of which also bind to cannabinoid receptors. Some ligands described to be GPR18 agonists or partial agonists include N-Arachidonoylglycine, Abnormal cannabidiol, O-1602, AM-251, Cannabidiol, delta 9-Tetrahydrocannabinol, N-arachidonoyl ethanolamine and Arachidonylcyclopropylamide. Antagonists include the synthetic cannabidiol related compounds CID-85469571 and O-1918.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: G protein-coupled receptor 18; G-protein coupled receptor 18; GPCRW; GPR18; N-Arachidonyl Glycine Receptor; NAGly Receptor
Gene Aliases: GPCRW; GPR18
UniProt ID: (Human) Q14330
Entrez Gene ID: (Human) 2841
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