|Immunohistochemistry (Paraffin) (IHC (P))||1:3000|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated Cys-LARAFGEEEFLSS-Abu-PRGNAPRE|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
IHC (P) analysis shows positive staining of GPR32 in human small intestine tissue neuroendocrine cells.
WB analysis shows GPR32 in glycoprotein-enriched fractions from GPR32 overexpressing 293 cells. Additional unknown bands at ~30kD and ~130kD were also detected.
While GPR32 has a theoretical MW of ~ 40kD, the observed band in a WB runs at ~60kD due to enrichment of the glycosylated receptor.
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. The intronless GPR32 gene encodes an orphan G protein-coupled receptor that binds to resolvin D1 and lipoxin A4. Resolvin D1 binding to GPR32 has been linked to resolution of pulmonary inflammation, suppression of TGFbeta induced epithelial mesenchymal transition of A549 lung cancer cells. Resolvins are being evaluated as new drug candidates acting at the resolution phase of chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: G protein-coupled receptor 32; G-protein coupled receptor 32; GPCRW; GPR32; Probable G-protein coupled receptor 32; Resolvin D1 Receptor; RVDR
Gene Aliases: GPR32; RVDR1
UniProt ID: (Human) O75388
Entrez Gene ID: (Human) 2854
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