|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to near N-terminus of human GRP78.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with bovine, chicken, canine, amphibian, mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. GRP78 is localized in the endoplasmic reticulum where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 78 kDa glucose-regulated protein; BiP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose-regulated protein, 78kDa; GRP-78; Heat Shock 70 kDa protein 5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Immunoglobulin heavy chain-binding protein
Gene Aliases: BIP; GRP78; HEL-S-89n; HSPA5; MIF2
UniProt ID: (Human) P11021
Entrez Gene ID: (Human) 3309
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