|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human GRP-94 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
Glucose regulated protein 94 (GRP94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP94 and other resident ER proteins including GRP78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 94 kDa; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; glucose regulated protein; GP96; gp96 homolog; GRP-94; GRP94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; stress-inducible tumor rejection antigen gp96; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen-1
Gene Aliases: ECGP; GP96; GRP94; HEL-S-125m; HEL35; HSP90B1; TRA1
UniProt ID: (Human) P14625
Entrez Gene ID: (Human) 7184
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