|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunoprecipitation (IP)||1-5 µg|
|Western Blot (WB)||10 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Full length recombinant human GAP.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA4-001 detects GTPase activating protein (GAP) from human, mouse, rat, bovine and non-human primate tissues.
MA4-001 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. Immunofluorescence staining of GAP in mouse fibroblast cells with MA4-001 results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. MA4-001 immunoprecipitates GAP that is complexed with the GAP related p190.
The MA4-001 antigen is full length recombinant human GAP. Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.
Reconstitute with PBS.
GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GAP; GTPase-activating protein; p120 RAS GTPase activating protein; p120-rasGAP; p120GAP; Ras GTPase-activating protein 1; Ras p21 protein activator; RAS p21 protein activator (GTPase activating protein RAS p21); RAS p21 protein activator (GTPase activating protein) 1; RAS p21 protein activator 1; rasGAP; triphosphatase-activating protein
Gene Aliases: BOS_8464; CM-AVM; CMAVM; GAP; GAPX; p120; p120GAP; p120RASGAP; PKWS; RASA; RASA1; RASGAP
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.