|Immunoprecipitation (IP)||10-20 µg/mg of lysate|
|Western Blot (WB)||1:10,000-1:40,000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of human Lectin, Galactoside-binding 7 within exon 4.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage conditions||4° C|
PA1-20164 detects Galectin-7 in mouse samples.
PA1-20164 has been successfully used in immunoprecipitation and Western blot procedures.
The PA1-20164 immunogen is a synthetic peptide corresponding to a portion of human Lectin, Galactoside-binding 7 within exon 4 (LocusLink ID 3963)..
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Gal-7; GAL7; Galectin-7; HKL-14; lectin, galactoside binding soluble 7; lectin, galactoside-binding, soluble, 7; LGALS7A; p53-induced gene 1 protein; PI7
Gene Aliases: GAL7; Galectin-7; LGALS7; LGALS7A; LGALS7B; PIG1
UniProt ID: (Human) P47929
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.