|Western Blot (WB)||1:1000-1:10,000|
|Tested Species reactivity||Luciferase|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant Gaussia luciferase expressed in E. coli|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-181 detects a ~20 kDa protein in the supernatant of Gaussia luciferase-secreting cells.
PA1-181 can be used for activity-independent verification of Gaussia luciferase expression.
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. Gaussia luciferase can be measured individually or in multiplex assays, such as the Gaussia-Firefly Luciferase Dual Assay Kit (Product # 16181). The Gaussia luciferase has been codon optimized for enhanced performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Gaussia luciferase; Gaussia princeps luciferase
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