|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/ml|
|Western Blot (WB)||1-1.25 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from a region between residues 272 - 321 of human Gli2.|
|Storage buffer||PBS with 2% sucrose|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-30042 detects Gli2 from human samples.
PA1-30042 has been successfully used in immunohistochemistry (paraffin tissue) and Western blot applications. Predicted molecular weight of 133 kDa.
The PA1-30042 immunogen is synthetic peptide derived from a region between residues 272 - 321 of human Gli2. Positive control of jurkat cell lysate for western blot applications and human heart and human intestine tissue for immunohistochemistry application suggested.
This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP1; THP2; Zinc finger protein GLI2
Gene Aliases: CJS; GLI2; HPE9; PHS2; THP; THP1; THP2
UniProt ID: (Human) P10070
Entrez Gene ID: (Human) 2736
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