|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Gel Shift (GS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:2,500|
|ChIP assay (ChIP)||See 3 publications below|
|Gel Shift (GS)||See 5 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Reptile, Rat|
|Published species reactivity||Rabbit , Rat , Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: A(150) P T E K E F P K T H S D V S S E Q Q H L K G Q T G(175)|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-510A detects glucocorticoid receptor (GR) from human, rat, and reptile tissues. This product detects both the unactivated and activated forms of the receptor.
PA1-510A has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract.
PA1-510A immunizing peptide corresponds to amino acid residues 150- 175 from human GR.
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
The corresponding gene for the glucocorticoid receptor is NR3C1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GCCR; GCR; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; Glucocorticoid receptor lymphocyte; GR; GRL; Grl1; NR3C1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
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