|Immunocytochemistry (ICC)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||5 µg/ml|
|Western Blot (WB)||See 32 publications below|
|ChIP assay (ChIP)||See 11 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|ChIP-sequencing (ChIP-Seq)||See 2 publications below|
|Immunocytochemistry (ICC)||See 7 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Immunohistochemistry (IHC)||See 14 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rabbit , Yeast , Rat , Non-human primate , Bovine , Sheep , Mouse , Human , Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR.|
|Purification||Antigen affinity chromatography|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-511A detects glucocorticoid receptor (GR) from human, mouse and rat tissues.
PA1-511A has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract. Immunocytochemical staining of GR in HeLa cells using PA1-511A results in cytoplasmic staining in the absence of ligand, and nuclear staining after hormone administration.
The PA1-511A immunogen is a synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR. The PA1-511A immunizing peptide (Cat. # PEP-001) is available for use in neutralization and control experiments.
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
The corresponding gene for the glucocoritcoid receptor is NR3C1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GCCR; GCR; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; Glucocorticoid receptor lymphocyte; GR; GRL; Grl1; NR3C1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
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