|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||1-5 ug|
|Western Blot (WB)||2-4 µg/ml|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Rat Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to amino acids 161-177 of the rat glutamate transporter EAAC1 sequence.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: EAAC1; EAAT3; excitatory amino acid carrier 1; Excitatory amino acid transporter 3; excitatory amino acid transporter-3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; SLC1A1; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; Solute carrier family 1 A1 (brain glutamate transporter); Solute carrier family 1 member 1; solute carrier family 1, member 1
Gene Aliases: DCBXA; EAAC1; EAAT3; REAAC1; SCZD18; SLC1A1
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