|Western Blot (WB)||1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, His-tagged, recombinant, human glutathione peroxidase 3 protein expressed in E. coli.|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is Bosc23 cells transfected withmyc-Gpx3.
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Extracellular glutathione peroxidase; Glutathione peroxidase 3; glutathione peroxidase 3 (plasma); GPx-3; GPx-P; GPX3; Plasma glutathione peroxidase
Gene Aliases: GPx-P; GPX3; GPXP; GSHPx-3; GSHPx-P
UniProt ID: (Human) P22352
Entrez Gene ID: (Human) 2878
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.