|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||1-2 µl|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human glutathione reductase protein expressed in E. coli.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is HeLa cells.
Glutathione reductase (GR) is a member of pyridine nucleotide-disulfide oxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG) : GSSG + NADPH + H+ to 2GSH + NADP+ In most eukaryotic cells, GR maintains the ratio of [GSH]/[GSSG] elevated, and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: epididymis luminal protein 75; epididymis secretory sperm binding protein Li 122m; glutathione reductase; Glutathione reductase, mitochondrial; glutathione S-reductase; GR; GRase
Gene Aliases: GLUR; GRD1; GSR; HEL-75; HEL-S-122m
UniProt ID: (Human) P00390
Entrez Gene ID: (Human) 2936
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