|Flow Cytometry (Flow)||1 ug/ml X 10^6 cells|
|Western Blot (WB)||2-3 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 26–38 of human Granzyme B|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Granzyme B is a serine protease contained within cytoplasmic granules produced by cytotoxic T lymphocytes and natural killer cells. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. It can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzyme B has also been shown to play an important role in activation induced cell death of T helper 2 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C11; Cathepsin G-like 1; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA-1; CTLA1; CTSGL1; cytotoxic serine protease B; Cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin 2; Fragmentin-2; granzyme 2; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GrB; HLP; Human lymphocyte protein; Lymphocyte protease; SECT; T-cell serine protease 1-3E
Gene Aliases: CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; GRB; GZMB; HLP; SECT
UniProt ID: (Human) P10144
Entrez Gene ID: (Human) 3002
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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