|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1:1,000|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length W79A human group V phospholipase A2.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-830 contains 100 ug of in vitro produced, protein A purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.
MA1-830 detects Group V Secretory Phospholipase A2 from human and mouse samples.
MA1-830 has been successfully used in Western blot procedures. By Western blot, MA1-830 detects a ~15 kDa band representing Group V Secretory Phospholipase A2 from mouse macrophages.
The MA1-830 immunogen is full length W79A human group V phospholipase A2.
This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Ca2+-dependent phospholipase A2; Calcium-dependent phospholipase A2; DKFZp686C2294; Group V phospholipase A2; GV-PLA2; hVPLA(2); MGC46205; Phosphatidylcholine 2-acylhydrolase 5; phospholipase A2, group V; PLA2-10
Gene Aliases: FRFB; GV-PLA2; hVPLA(2); PLA2; PLA2-10; PLA2G5; sPLA2
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