|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues E A N N L G L L D P N.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-1756 detects HC2 from human and mouse samples.
PA1-1756 has been successfully used in immunohistochemical staining of paraffin embedded tissue sections.
The PA1-1756 immunogen is a synthetic peptide corresponding to residues E A N N L G L L D P N. This immunizing peptide (PEP-302) is available for neutralization and control experiments.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1; alpha type; inter-alpha (globulin) inhibitor H2; inter-alpha (globulin) inhibitor, H2 polypeptide; inter-alpha-inhibitor heavy chain 2; Inter-alpha-trypsin inhibitor complex component II; Inter-alpha-trypsin inhibitor heavy chain H2; ITI heavy chain H2; ITI-HC2; macropain) subunit; proteasome (prosome; PSMA1; Serum-derived hyaluronan-associated protein; SHAP
Gene Aliases: AI747202; H2P; IGHEP2; Intin2; Itih-2; ITIH2; SHAP
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