|ChIP assay (ChIP)||1-3 ul|
|Flow Cytometry (Flow)||1-20 µg/ml|
|Immunocytochemistry (ICC)||1.0 µg/ml|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||0.4 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.5 µg/ml|
|Western Blot (WB)||See 6 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Dog, Hamster, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-861 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells.
PA1-861 has been successfully used in Western blot, ChIP, immunoprecipitation, immunofluorescence, flow cytometry, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing HDAC2 and a ~40 kDa protein of unknown identity from HeLa cell lysate. Immunocytochemical staining of HDAC2 in HeLa cells with PA1-861 results in nuclear staining.
The PA1-861 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HD2; Histone Deacetylase 2; transcriptional regulator homolog RPD3; YY1 transcription factor-binding protein; YY1-associated factor 1
Gene Aliases: D10Wsu179e; HD2; HDAC2; mRPD3; RPD3; YAF1; Yy1bp
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