|ChIP assay (ChIP)||1-3 ul|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1,000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1)SSQSHPDGLSGRDQPVE(18) of human HDAC4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-863 detects histone deacetylase 4 (HDAC4) from human cells.
PA1-863 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~140 kDa protein representing HDAC4 from HeLa cell lysate. An ~110 kDa protein is also detected with PA1-863 which could correspond to protein degradation product.
PA1-863 immunogen is a synthetic peptide corresponding to residues M(1)SSQSHPDGLSGRDQPVE(18) of human HDAC4.
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.
HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with N-CoR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HD4; Histone Deacetylase 4; histone deacetylase A
Gene Aliases: AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDAC4; HDACA; KIAA0288
UniProt ID: (Human) P56524
Entrez Gene ID: (Human) 9759
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