|Immunohistochemistry (IHC)||Assay dependent|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Western Blot (WB)||1:2000-1:6000|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Non-human primate Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||GST-HEF-1 (82-398 aa.) from SP2/0 cells|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-5784 detects HEF1 in human, mouse, and rat samples. This antibody does not recognize p130cas. It has not been tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites.
MA1-5784 has been successfully used in Western blot, immunoprecipitation, immunohistichemistry, and immunofluorescence procedures. For IHC, it is recommended to use human kidney as a positive control.
The MA1-5784 immunogen is GST-HEF-1(82-398aa.).
Store at 4°C for one month or at -20°C for longer periods.
HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cas scaffolding protein family member 2; CAS-L; cas-like docking; CASL; Crk-associated substrate lymphocyte-specific protein; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF1; mEF1; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated gene 9; p105; p130Cas-related protein; Renal carcinoma antigen NY-REN-12
Gene Aliases: CAS-L; CAS2; CASL; CASS2; HEF1; MEF1; Nedd-9; NEDD9; p105
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.