|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues in human HIC2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 72 kDa.
Purity is >95% by SDS-PAGE.
HIC-2 (hypermethylated in cancer 2) possesses zinc finger motifs that are thought to be important for DNA-binding and also has a BTB/POZ domain at the N-terminus, which is thought to be important for protein-protein binding, as well as for the binding of transcription factors. HIC-2 is also known as Hic-3, HIC1-related gene on chromosome 22 or Zinc finger and BTB domain-containing protein 30, and is a 615 amino acid protein that is expressed as two isoforms produced by alternative splicing. HIC-2 is highly expressed in cerebellum and is localized to the nucleus in cells. HIC-2 contains a short amino acid sequence that is thought to interact with CtBP, a transcriptional repressor. The gene sequence associated with HIC-2 is thought to be a target for miRNAs (microRNAs) which are expressed in many cancers, suggesting that HIC-2 could possess tumor suppressor capabilities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Hic-2; Hic-3; HIC1-related gene on chromosome 22 protein; HIC1-related gene on chromosome 22 protein Hic-3; HIC2; HRG22; Hypermethylated in cancer 2 protein; KIAA1020; ZBTB30; Zinc finger and BTB domain-containing protein 30
Gene Aliases: AA409108; HIC2; HRG22; KIAA1020; mKIAA1020; ZBTB30; ZNF907
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