|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Gel Shift (GS)||1:1-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Western Blot (WB)||1:500|
|Immunohistochemistry (IHC)||See 27 publications below|
|Western Blot (WB)||See 12 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Gel Shift (GS)||See 3 publications below|
|Tested Species reactivity||Bovine, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast|
|Published species reactivity||Rabbit , Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Fusion protein containing residues 432-528 of human HIF-1alpha.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.
For WB, testing on nuclear extracts is recommended.
Suggested positive control: Cos7 CoCl2-treated nuclear extract.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; Hypoxia Inducible Factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8
Gene Aliases: AA959795; BHLHE78; BOS_11009; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8
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