|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Bovine, Ferret, Human, Mouse, Non-human primate, Sheep, Rat|
|Host / Isotype||Mouse / IgG1, Kappa|
|Immunogen||Fusion protein containing amino acids 496-789 of human HIF-1 beta.|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ARNT protein; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; beta subunit; bHLHe2; Class E basic helix-loop-helix protein 2; dioxin receptor; Dioxin receptor, nuclear translocator; HIF-1 beta; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; hypoxia-inducible factor 1; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; nuclear translocator; TANGO
Gene Aliases: ARNT; Arnt1; BHLHE2; BOS_2904; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO; W08714
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